Characterization of a prokaryotic SMC protein involved in chromosome partitioning.

نویسندگان

  • R A Britton
  • D C Lin
  • A D Grossman
چکیده

smc of Bacillus subtilis encodes a homolog of eukaryotic SMC proteins involved in chromosome condensation, pairing, and partitioning. A null mutation in B. subtilis smc caused a temperature-sensitive-lethal phenotype in rich medium. Under permissive conditions, the mutant had abnormal nucleoids, approximately 10% of the cells were anucleate, and assembly of foci of the chromosome partitioning protein Spo0J was altered. In combination with a null mutation in spo0J, the smc mutation caused a synthetic phenotype; cell growth was slower and approximately 25% of the cells were anucleate. Our results demonstrate that the B. subtilis Smc protein, like its eukaryotic counterpart, plays an important role in chromosome structure and partitioning.

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عنوان ژورنال:
  • Genes & development

دوره 12 9  شماره 

صفحات  -

تاریخ انتشار 1998